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Islets were harvested by handpicking under a microscope and cultured in a cell culture incubator (37C and 5% CO 2 ) in DMEM containing 11.1 or 22.2 mmol/l glucose, 1 mmol/l pyruvate, non-essential amino acids, 100 U/ml penicillin, 100 g/ml streptomycin, 2 mmol/l glutamine, 50 g/ml gentamicin, 10 g/ml fungison and 10% FCS, with or without 1 mmol/l cystine

The treatments were performed for 72 h, and for the final 6 h of treatment, H 2 O 2 substrate solution was added to the wells, and 2 h before the end of the treatment, 100 L of ROS-Glo detection solution was added to each well