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Description

Cleaved caspase-3 activity assay A cleaved caspase-3 ELISA kit (Cell Signaling Technologies, USA) was used to measure endogenous levels of cleaved caspase-3 protein in vivo and in vitro

Percentages of sperm cells with lipid peroxidation and intracellular ROS were analyzed using a FACS-Calibur flow cytometer (Becton Dickinson, USA)

Reaction: Homocysteine + Methylcobalamin Methionine + Tetrahydrofolate Importance: Methionine synthase plays a critical role in maintaining methionine levels for protein synthesis and SAM synthesis