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J Neurol Neurosurg Psychiatry 2020;91:622630.ArticlePubMedPMC 32

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Briefly, cells were grown in YPD until logarithmic phase, harvested by centrifugation at 1,000 g for 3 min at room temperature and resuspended in 200 l One-step-transformation buffer containing 40% polyethylene glycol 3,350 (Sigma Aldrich, St