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Description

No oxygen is consumed, nor is CO 2 produced in this pathway

To determine the amount of NFT required after its initial addition and a washout phase to maintain elevated glutathione levels, cells were pretreated with 20 M NFT for 48 h, maintained in NFT-free medium for 24 h, and subsequently treated with varying concentrations of NFT over 24 h

Treatment begins with 0.25 mg administered subcutaneously once weekly for four weeks, serving primarily as a tolerance-building dose rather than a therapeutic one