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Table 6 Changes in the expression of genes in Acidithiobacillus caldus MTH-04 in gr knockout and gr overexpression strains under zinc stress

Cell Viability Assays Cell were treated in 96-well plate for 48 h and incubated with 5 mg/ml 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) for 4 h at 37 C in the dark