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Protein content was determined using the Bio-Rad DC Protein Assay, and 510 g aliquots of protein were loaded on a ClearPAGE SDS Gel (CBS Scientific, Del Mar, CA, USA) after addition of 50 mM dithiothreitol and 0.05% bromophenol blue

As per the general manifestation, since its minor items were closer to constitutional symptoms, such as myalgia, arthralgia/arthritis, fever, and weight loss, rather than those limited to a specific organ ( In the present study, serum GPX-3 concentration at diagnosis could not predict poor outcomes during follow-up in patients with AAV

Two-day cardiopulmonary exercise tests (CPET) have practically demonstrated this reduced anaerobic threshold in ME/CFS (393, 394)

10.1016/s0076-6879(85)13050-8 Methods Enzymol