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5.8 Flow cytometry To verify intracellular expression of antigens by the pDNA3.1 plasmid human embryonic kidney cells (293T) were cultured in six-well plates in RPMI-1640 medium (R0883-500ML, Sigma-Aldrich) supplemented with 10% heat-inactivated FCS, 2mM L-glutamine, and 100 U/ml penicillin-streptomycin at 37C until they reached 70-80% confluency

This is because it is the active form of the vitamin, and the body can use it immediately

When traveling, patients should use insulated medication travel cases with gel packs specifically designed for temperature-sensitive medications

Prophylaxis and treatment of other macrocytic anaemias associated with Vitamin B12 deficiency.