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Histological examinations of the liver and kidneys showed no lesions, and no abnormalities were detected in the gastrointestinal tract, such as congestion or hemorrhage

Gene expression analysis by reverse transcriptase-quantitative real-time PCR (RT-qPCR) After the homogenization step by using a TissueLyser II (Qiagen, Hilden, Germany) in the gastric tissue, total RNA was isolated using a commercial kit (12183018A PureLink RNA Mini Kit, Thermo Fisher) in accordance with the manufacturers recommendations