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OXY could also decrease pro-inflammatory cytokines levels and boost anti-inflammatory by downregulating TLR4, MyD88, NF-B, and PK2/PKR1 pathways, which are linked to LPS-induced orchitis

To stain phospho-(T94)Tctex-1, RPE-1 cells were first incubated with 0.1% Triton X-100supplemented PHEM buffer (100 mM PIPES, 100 mM Hepes, 2 mM MgSO 4 , 0.2 mM EDTA, and 4 mM EGTA, pH 6.9) for 30 s at room temperature before 4% PFA fixation for 10 min