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One approach compares mice of different genotypes (Cre+/ or LoxP+/), all of which are treated with TAM

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Two molecules of acetyl-CoA condense to form HMG-CoA, which is then reduced to mevalonate (MVA) by the rate-limiting enzyme HMGCR with NADPH consumption, and subsequently converted to cholesterol in the endoplasmic reticulum via squalene and lanosterolan energy-intensive process requiring ATP and NADPH ( Figure 1 )