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The second GFP tagging vector (PL_HC_026) was generated by a recombinase reaction between a modified Plasmo GEM vector carrying a gRNA cassette targeting the myoA gene and 3UTR with PL_HC_026 (Supplementary Fig

[DOI] [PMC free article] [PubMed] [Google Scholar] Ricciotti E., FitzGerald G.A

Ellmans assay Free glutathione after plasma treatment was observed using the glutathione detection kit commercially available from Enzo Life Science (#ADI-900-160) without glutathione reductase resulting in a typical Ellmans end point detection of free thiols, which react stoichiometrically with 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) by cleaving the disulphide bond yielding 2-nitro-5-thiobenzoate (TNB - )

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