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System of glutamine production (200 mL) by GS enzyme consists of TrisHCl (100 mM, pH 8.0), 100 mM L-glutamate, 100 mM (NH 4 ) 2 SO 4 , 50 mM polyP 6 , 5 mM ATP, 50 mM MgCl 2 , 5 U/mL PPK, 6 U/mL GS

Methods Enzymol 252:2630 Meister A, Anderson ME (1983) Glutathione

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