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Notably, the severe flooding in 2022 highlighted the districts vulnerability, which stems from its low-lying geography, insufficient green spaces, and aging drainage systems (Park et al., 2024

NRF2 directly upregulates genes associated with iron metabolism (HO-1, ferritin light and heavy chain) and antioxidant activity (GPX4, thioredoxin reductase 1, GCLC, GCLM, and the cystine/glutamate antiporter SLC7A11), which prevent free iron accumulation and lipid peroxidation

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The samples were then quickly frozen and thawed in liquid nitrogen for 3 cycles, centrifuged at 12,000 rpm for 5 min at 4 C and the supernatant was collected