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Facile preparation of a pH-sensitive biocompatible nanocarrier based on magnetic layered double hydroxides/Cu MOFs-chitosan crosslinked -carrageenan for controlled doxorubicin delivery to breast cancer cells

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The balanced increase of GSH and GSSG levels in the Cpz 1 group can be explained by the activation of glutathione peroxidase-related way of the destruction of H 2 O 2 coupled with the oxidation of the GSH ( Importantly, the indicated features of the oxidative changes in the present study are similar to that reported early for the freshwater bivalve mollusc Unio tumidus subjected to Cpz in the concentration of 18.0 g L -1 for 14 days ( Phenothiazine and its derivatives including Cpz have been known for many years as potent antioxidants, based on its reducing activity via the formation of radical cation, in the interaction, for example, with the thiyl radical derived from GSH ( Carassius auratus , acute toxicity of Cpz indicated as the median lethal concentration (LC 50 ) in 24, 48 and 96 has 1.11, 0.43 and 0.32 mg L -1 correspondingly, was accompanied with the oxidative manifestations different depending on the time of exposure ( Some signs of Cpz pro-oxidative effects were confirmed in the erythrocytes from human blood treated with Cpz in concentrations observed in vivo at therapeutic doses (1100 M) for 2 hours ( -1 ) induced the antioxidant enzyme SOD, but did not affect catalase, and inhibited lipid peroxidation ( Hence, our results confirm that the impact on the antioxidant-pro-oxidant balance is the intrinsic feature of Cpz toxicity

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