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Preparation of mitochondria Mitochondria were prepared from 180200 g Wistar rat liver using a standard protocol from Clayton and Shadel (Clayton and Shadel 2012)

doi: 10.3390/ijms24010449 44 ZhangYLuYJinL

170 , e61809 (2021)

Gene Expression Explants were collected from each medium group at day 7, flash frozen in liquid nitrogen, and stored at -80C for analysis of gene expression (n=3-7/group)