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Description

91 LevineM.Conry-CantilenaC.WangY.WelchR

Glutathione standards of 0, 2.5, 5, 10 M and 100 M of both GSH and GSSG were prepared and combined in the following ratios to a final volume of 90 L: 10 M GSH: 0 M GSSG, 9 M GSH: 1 M GSSG, 8 M GSH: 2 M GSSG, 6 M GSH: 4 M GSSG, 4 M GSH: 6 M GSSG, 2 M GSH: 8 M GSSG, 1 M GSH: 9 M GSSG, and 0 M GSH: 10 M GSSG

Primary antibodies were incubated for 30, 60 or 120 min

Increasing evidence highlights natural polysaccharides as promising therapeutic agents due to their dual roles in microbiota modulation and barrier reinforcement