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Genetic studies reveal the role of the endocrine and metabolic systems in aging

Metabolomics profiling of mouse plasma, MRS medium, and bacterial supernatants Ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) analysis Four volumes of 80% methanol in water were added to 20 L of mouse plasma, bacterial culture supernatants, or MRS media

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Additionally, these substances depleted ATP levels, impaired mitochondrial function, reduced cell proliferation, and induced autophagy, necrosis, and apoptosis 50