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Given that the majority of patients with breast cancer predominantly exhibited POI during chemotherapy, and considering that there were differences in the internal microenvironment induced by breast cancer and the normal internal microenvironment, we simulated the dosage and duration of chemotherapy to establish a breast cancer mouse model of POI 41,42,43

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Friedrich M, Pohin M, Jackson MA, Korsunsky I, Bullers SJ, Rue-Albrecht K, et al

Upon dryness 200 L of 3N butanolic HCl was added, and samples were derivatized at 65C for 90 minutes to promote butyl esterification, evaporated to dryness under nitrogen at 35C, and reconstituted in a solvent matrix of acetonitrile/deionized H 2 O (50/50 v/v) with 0.1% formic acid