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For macrophage polarization experiments, cells were then treated with either human recombinant IL-4 (10 ng/mL, Cedarlane Labs, Burlington, ON) or IFN- (10 ng/mL, eBioscience, San Diego, CA) for 48 h

Besides, valine was reported to improve liver diacylglycerols, and reduce NASH histology with profound hepatoprotective effects on oxidative stress and inflammatory proteins ( Fusobacteriota and Deferribacterota) abundances and revealed the adverse metabolic response to NAFLD, which suggested reducing dietary valine as a new approach to preventing NAFLD of laying hens ( Prevotellaceae_UCG-001 which was positively correlated with valine, was decreased in NASH and increased by LGZG

Serum bile acid levels and gene expression were modulated in a circadian-dependent manner, consistent with Linafexor's short half-life and metabolic rhythms

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