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The fluorescence quenching values, (F 0 F)/F 0 , showed a linear dynamic range for the concentrations of GSH from 0.25 to 16 M, which is sensitive to detect the milli-molar concentrations of endogenous GSH in most mammalian cells

Paired with Cys596, this residue directly participates in enzymatic catalysis together with FAD and at least two other cystine pairs (Cys28-SS-Cys31 and Cys154-SS-Cys159) with redox activity 29