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The culture medium was replaced at each time-point and the cells were then incubated with 110 l complete medium containing 10 l Cell Counting Kit (CCK)-8 reagent (Beijing Solarbio Science & Technology Co., Ltd.) for 90 min at 37C

Minoxidil is a well-known and effective treatment for hair loss, but many people may consider stopping it for different reasons, such as: Limited or slower-than-expected results Side effects and discomfort Cost of continuous Concerns about long-term use and/or interactions with other medications Feeling theyve achieved their desired results It is important that if you are considering stopping, please speak with your hair loss Doctor or hair transplant Surgeon as you need to consider the impact on your hair loss

10.1002/adfm.201908865 44 Luis Garcia-GimenezJ.MarkovicJ.DasiF.QuevalG.SchnaubeltD.FoyerC

To view a copy of this license, visit About this article Cite this article Chen, J., Li, X., Ge, C