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Disclosures: Chinmay Raut: Nothing to Disclose, Rawdat Hussain: Nothing to Disclose, Akram Irshaid: Nothing to Disclose, Prabhu Ponnandy: Nothing to Disclose, Elizabeth Speliotes: Nothing to Disclose 2558 GENOME-WIDE CRISPR KNOCKOUT GENETIC SCREEN IN LIVER CELL LINES TO IDENTIFY GENES AFFECTING HEPATIC STEATOSIS Prabhu Ponnandy 1 Chinmay Raut 1 Rawdat Hussain 1 Akram Irshaid 1 Brian Halligan 2 Elizabeth Speliotes 2 , 1 Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of Michigan, 2 University of Michigan Medical Center Background: MASLD affects~30% of the adult population worldwide, with its prevalence increasing from 22% to 37% from 1991 to 2019

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