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Sigma-Aldrich, United Kingdom) for 1 h at room temperature, and subsequently incubated with primary antibodies (tri-methyl histone H3 (Lys4) rabbit mAb, ubiquityl-histone H2A (Lys119) rabbit mAb, phospho-CaMKII (Thr286) rabbit mAb, phospho-CREB (SER133) rabbit mAb, WDR5(A-6), anti-BDNF antibody, anti-beta-actin) at 4C overnight