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In all our previous murine studies we have used short cycles (1014 days) of very low subcutaneal doses (20 g/kg/day) after carefully assessing its circulating concentrations and using the minimum amount that was sufficient to adequately restore physiological values of the molecule

In a transgenic mouse expressing low GSH levels (Gclm KO), we found reduced number of prefrontal and hippocampal PV-immunoreactive interneurons together with diminished high-frequency neuronal synchronization [81, 82], reduced number of PV-immunoreactive neurons in thalamus reticular nucleus together with altered firing properties [56], thus demonstrating the functional consequence of a redox dysregulation

The batch of the ARPE-19 cells used in this study was validated using short tandem repeat (STR) analysis by Cobioer Biosciences (Nanjing, China)

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