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Q.YangW.et al

Clear saliva samples (about 1 ml) were immediately aliquoted to sterile 0.2 ml micro test tubes, Eppendorf type, and frozen at -80C until assayed

Although one may assume daily cycles in DNA methylation could be driven by daily cycles in cell proliferation and associated DNA replication, slow proliferating cells of the liver, lung, and brain in mice also exhibit daily cycles in methylation, particularly at CpG sites [89,90,91]