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doi:10.1186/s12916-022-02328-x Jovanovski E, Yashpal S, Komishon A, et al

Acylcarnitine assay Acylcarnitines were extracted from either 10 l of whole liver homogenate, 50 l of plasma, 50 l brain parenchyma, 50 l cerebrovasculature (timepoint study), 60 l of cerebrovasculature (cerebrovascular uptake study) or 100 l of brain homogenate (all homogenized as described above) and spiked with 5 l of acylcarnitine internal standard (IS) mix containing 12 M TMAO-d9 (Cambridge Isotope Laboratories, Inc., CIL), NSK-B (CIL) and NSK-B-G1 (CIL) at the beginning of the extraction process

Indeed, although IIEF-6 is a validated questionnaire distinguishing between presence or absence of ED by considering the whole spectrum of aspects related to penile erectile function, and also grading the severity of ED in clinical setting, it still remains a subjective tool, prone to the risk of recall bias, patients emotional drawbacks or patients conditioning due to uneasiness or misinterpretation of questions

Facts About the Current Good Manufacturing Practices (cGMPs)