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They are released when bacteria die and are not destroyed by standard sterilization methods autoclaving kills the organisms but leaves the endotoxin fragments intact

Some common methods used for gene manipulation and their applications in Drosophila research are as follows: Mutant alleles LOF mutant alleles can be induced through various techniques, including chemical mutagenesis, radiation-induced mutagenesis, or more precise methodologies like CRISPR/Cas9 genome editing (Obe et al., 1971