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When freezing your Trimix, its important to remove it from the freezer and let it thaw completely at room temperature

Then 20% sodium dodecyl sulfate/50% N,N-dimethylformamide (both from Fisher Scientific) solution in milliQ water (EMD Millipore) was added 1:1 to the cell culture in each well to lyse the cells and solubilize the formazan crystals