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10.1073/pnas.1410104111 Proc

Full details are provided in the Supplemental Methods

Methylcobalamin may appeal to those avoiding the trace cyanide moiety in cyanocobalamin (which is considered safe at supplement doses) or who prefer active forms

Peptides were then on-line eluted into a Fusion Tribrid mass spectrometer (Thermo Scientific, San Jose, CA) from an EASY PepMap TM RSLC C18 column (2m, 100, 75m x 50cm, Thermo Scientific, San Jose, CA), using a hold of 6% solvent B (80/20 acetonitrile/water, 0.1% formic acid) for 15 minutes, followed by a gradient of 6-28% solvent B in 105 minutes, then 28-49% solvent B in 15 minutes, 40-100% solvent B in 10 minutes, a 10 minute hold of 100% solvent B, a return to 5% solvent B in 3 minutes, and finally a 3 minute hold of 5% solvent B