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Briefly, mice were anesthetized (isoflurane inhalation 2.5% for induction 2% for maintenance) and dorsal hair was removed by electric razor and skin was disinfected with chlorhexidine gluconate (0.5% w/v) in a 70% v/v ethanol solution

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Garzon R, Wang M , Kuruvilla J, Gutierrez M, Savoie L, Mau-Srensen M, Flinn I, Baz R, Martin P, Gabrail N, Goy A, Brown P, Rashal T, Carlson R, Elloul S, Friedlander S, Saint-Martin J, Frenkel R, Raza-Mirza M, Norori S, Shacham S, Kauffman M, Wagner-Johnston N