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Cell viability was determined using the CyQUANT Direct Cell Proliferation Assay Kit (Thermo Fisher, Waltham, MA) and Alamar Blue Cell Viability Reagent (Thermo Fisher) according to the instruction of each manufacturer

Iron homeostasis is further modulated by the IRP-IRE signaling pathway, where iron regulatory proteins (IRP1/2) bind to iron-responsive elements (IREs) in the untranslated regions of ferritin and ferroportin (FPN) mRNAs