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This chromatin mark is established on unmethylated CpG island genes early in development and then maintained in differentiated cell types by the presence of an EZH2-containing P complex

10.3791/51439 J

As for the role that caffeine has in the purported benefits of the coffee enema, drinking coffee is a far more effective method for increasing the amount of caffeine in your blood (and hence causing vasodilation to potentially send toxins from the blood into the colon, or causing increased bile acid secretion to potentially facilitate liver detoxification) compared to administering it at the other end

10.1074/jbc.ra118.003129 213 ZhaoY.HuQ.ChengF.SuN.WangA.ZouY.et al (2015)