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Assessment of meiotic spindle configuration Mature oocytes were denuded and fixed with methanol (1:1) in PBS for 20 min at room temperature and stored in PBS-PVA at 4 C until use

This respiratory burst is essential for the destruction of pathogens [47]

Samples were run on either a SeQuant ZIC-pHILIC LC column, 5 m, 150 4.6 mm (MilliporeSigma, Burlington, MA) with a SeQuant ZIC-pHILIC guard column, 20 4.6 mm (MilliporeSigma, Burlington, MA) or an Atlantis Premier BEH Z-HILIC VanGuard FIT column, 2.5 m, 2.1 mm 150 mm (Waters, Milford, MA)