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For the IFN-I bioassay, 5 x 10 4 3T3 cells containing an interferon-sensitive response element (ISRE) fused to luciferase 51 were plated per well into 96-well white-bottom plates (Greneir 655083) in DMEM containing 10% FBS, 100 U/ml penicillin and 100 g/ml streptomycin

These findings classify NPC as a genetically modified, virus-related cancer and highlight the necessity for population-specific genetic risk assessment, EBV / HPV-derived biomarkers, and targeted preventative efforts

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