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The protein concentration was measured using Nanodrop (protein A280) and samples were normalized and the final concentrations of each sample and sample buffer were calculated and 5x loading dye was added

Hif-1alpha is an essential mediator of ifn-Gamma-Dependent immunity to mycobacterium tuberculosis

This research was funded by the National Training Program of Innovation and Entrepreneurship for Undergraduates (202410730173), the Fundamental Research Funds for the Central Universities of China (lzujbky-2024-1) and the Scientific Research Project of Health Industry in Gansu Province (GSWSKY2023-15)

In our previous study, we inactivated the CPD by covalently conjugating the Cys144 with a peptide and found that the fusion protein, Endo-F3 (D165A)-CPD inactive , is stable in the presence of InsP 6