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Zhao R, Yu T, Li J, Niu R, Liu D, Wang W

A.ThiruchelvamM

In the previous study, we had successfully generated FGF5 -knockout heterozygous sheep using the CRISPR/Cas9 system by direct one-step cytoplasmic injection of Cas9 mRNA and sgRNA into zygotes, and the relationship between FGF5 gene and wool length in sheep was proved 22