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To allow comparable reduction kinetics, the proteins were pre-oxidized with 10 mM H 2 O 2 for 30 min

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NRF2s transcriptional activity requires its nuclear localisation and NRF2 is also degraded through binding to KEAP1, a process counteracted by competitive interaction of the latter protein with PALB2 and/or SQSTM1

In previously published protocols different types of derivatization reagents (CMQT or CMLT), different pH of the buffer used in the protocols (7.4) and shorter time of sample reduction and derivatization (3 min) were applied (Tables 3 and 4.) Changing the conditions of reduction and derivatization increases the efficiency of this process, which may give higher final concentrations of the analytes