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J Clin Endocrinol Metab 98(3):10311039

Biotin injections are most beneficial for people with medical issues that cause biotin deficiency, such as: Skin rashes Diabetes Nerve pain Alopecia Other related underlying causes of hair loss or brittle nails Injections are more effective than oral biotin supplements when immediate results are desired because they allow for maximum absorption of the vitamin directly into the body

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RING domain was originally thought to mediate DNA binding, but numerous studies since 1999 unambiguously establish that most RING domain proteins possess E3 activity (Deshaies and Joazeiro 2009)