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Different from heart and endothelial cells, smooth muscle cells contain abundant ZIP6 in addition to ZIP1 and ZIP13 as zinc influx transporters, while ZnT1, ZnT5, and ZnT9 are abundant as zinc efflux transporters (Figure 1C) 45

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In this work, scalable methods were investigated for the manufacture of an oncolytic virus immunotherapy application consisting of a prototype strain of coxsackievirus A21 (CVA21) produced in adherent MRC-5 cells