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Importance of the omega-6/omega-3 balance in health and disease: evolutionary aspects of diet

The RTqPCR mixture consisted of 5 L of SYBR Green Master Mix (Yeasen, Shanghai, China), 1 L of upstream primer, 1 L of downstream primer (with a final concentration of 0.2 M for each primer), and 2 L of cDNA, with enzyme-free water added to a final volume of 10 L

MitoNEET is an iron-containing outer mitochondrial membrane protein that regulates oxidative capacity

At the structural level, Glo-1 immunoreactivity in astrocytes was observed to be present the cell body as well as in the main astrocytic processes, i.e., along the GFAP-positive filaments