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The concentration of trichloroacetic acid should not exceed this value to avoid complicating subsequent staining of samples in the reaction with Ellmans reagent [9]

Note: cooking collagen turns it to gelatine, which provides the body with amino acids, the building blocks of proteins

Luminescence measurement was performed by multi-mode microplate reader (FLUOstar Omega)

Immune-related pathways, including T-cell selection/differentiation and Toll-like receptor signaling, were negatively enriched in ASD cohorts relative to age-matched pediatric healthy groups (Supplementary Fig