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Without intrinsic factor, the small intestine cannot efficiently absorb dietary B12 through the normal receptor-mediated pathway

A 2025 JAMA Neurology trial (n=1,500) demonstrated a 15-20% reduction in Hamilton Depression Rating Scale (HDRS) scores in deficient adults (serum B12 200 pg/mL and methylmalonic acid (MMA) 0.4 mol/L), with higher doses compensating for poor absorption or severe symptoms like megaloblastic anemia and neuropathy

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Key Points About Vitamin B12 and Urine Color: The effect of B12 on urine color can depend on the dosage and individual metabolism