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Insulin and insulin-like growth factor stimulation of vascular endothelial growth factor production by luteinized granulosa cells: comparison between polycystic ovarian syndrome (PCOS) and non-PCOS women

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Cells were lysed for 20 minutes in buffer containing: 50 mM HEPES pH 7.4, 40 mM NaCl, 2 mM EDTA, 0.5% Triton X-100, 1.5 mM sodium orthovanadate, 50 mM NaF, 10 mM sodium pyrophosphate, 10 mM sodium -glycerophosphate, and protease inhibitor tablet (Roche, Nutley, N.J.)

In order to assess the influence of the complications associated with CKD in this study, cats with CKD were identified as having proteinuria, hypertension, and anemia