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After two washes with pre-warmed PBS, cells were fixed with 4% PFA for 10 min at room temperature and EdU incorporation was visualized using the Click-iT EdU kit (Invitrogen), following the manufacturers protocol

This term refers to the proportion of a substance that enters the circulation when introduced into the body and is so able to have an active effect

To characterize the differentially expressed genes between the oxidized and reduced populations, DESeq2 was used to identify genes that were specifically upregulated in each category with respect to the other category using the above criteria

From these analyses, the concentrations of Glu and PyroGlu can be correlated with clinical outcomes