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Intramuscular injection may be clinically preferable to oral supplementation in specific situations: For individuals whose bloodwork shows deficiency or insufficiency in B12, vitamin D, or other measurable nutrients, and who have not achieved adequate levels through oral means

Plant-based diets contain no natural B12

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Similarly, substrate nicotinamide at 100 M, methyl donor S-adenosyl-L-methionine (SAM) at 5 M, and S-adenosyl methionine (SAH) at 5 M concentration) samples were also run individually in the reaction buffer [1 mM Tris [pH 8.6], 1 mM DTT, 10% trichloroacetic acid, 4% methanol, and water] to identify elution time and define substrate, co-factor, and product peaks