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Diagnosis is made by exclusion of alcohol abuse and other causes of liver disease (HBV-related hepatitis, HCV-related hepatitis, autoimmune liver diseases, polycystic ovary syndrome, drug-induced liver disease and congenital causes such as hereditary hemochromatosis, Wilsons disease, alpha-1 antitrypsin deficit)

On the day of the assay, plates were removed from the incubator and each well was washed twice with 100 l HBSS (Gibco, Life Technologies) and pre-incubated for 30 min at 37 C with 85 l HBSS per well

Selank Supplies Needed Plan supplies based on the SubQ schedule above: one daily injection at 250-500 mcg from a 30 mg vial