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Description

127 Russell, I

Preparation of single-cell suspension from tissue Foot skin Whole foot skin was cut into small pieces, which were digested for 45 to 60 min at 37 C under gentle agitation in RPMI 1640 containing 0.2 mg/mL collagenase P and 0.1 mg/mL DNAse I (both Roche Diagnostics GmbH)