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Determination of the mtDNA copy number Relative mtDNA copy numbers were determined by qPCR using the amplification curves of a gene encoded on the mtDNA ( MT-ND1 , NC_012920.1) and a single copy gene on the nuclear DNA ( NDUFV1 , NM_001166102.2) using oligonucleotide primers (see Supplemental Table S2) with the SYBR green technology [22] on a TRIO combi cycler (Biometra, 846-2-070-720)