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Measurement of ALDH2 enzymatic activity in mouse brain The mice were administered ALA (20 mg/kg) concomitantly with 4-MP (10 mg/kg), and 90 min later, the brain total fractions of the mitochondrial and cytoplasmic proteins were separated as described previously (Bunik et al., + , 50 mM FA and 20 l of the brain mitochondrial fraction