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Indeed, there was a positive correlation between glycemic level and WBC ROS accumulation only in LOsG-treated animals ( r = 0.61, n = 14, p 0.05)

Excess media was removed and replaced with 70% L15 media containing 10 M DCFH-DA and incubated at 20C for 30 min (32), after which the solution was removed, replaced with 10 g/ml Calcofluor White (CFW) to stain Bd for 5 mins (28), rinsed and replaced with 70% L15 media

Remarkably, TZDs performed an ability to suppress ferroptosis though selectively inhibiting ACSL4 and reduce mortality in kidney-specific Gpx4 knockout mice (193, 194)